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Cellular Screening

Orthogonal screens that create physiologically relevant insights into your therapeutic

Cellular screening helps bridge the gap between early-stage biochemical and biophysical screening and more customized phenotypic assays employed during the lead optimization stages of drug discovery.

These assays provide a biologically relevant environment to evaluate how compounds or biologics interact with cellular components such as receptors, enzymes and signaling pathways. By incorporating advanced imaging and automation, our cellular assays have delivered detailed phenotypic data, helping to identify lead compounds more efficiently and accurately.

We use cell-based screening assays to study how compounds influence parameters such as cell viability, proliferation, apoptosis, motility, changes in morphology or specific cellular functions. This is an essential step in the understanding of how a drug may behave in a complex biological environment, considering factors such as cell permeability, metabolism, and potential off-target effects. 

Our cell-based assays serve as a potent tool in the drug discovery process, offering target-agnostic and therapeutic modality-agnostic applications. Using our state-of-the-art liquid handling systems, and being co-located with our chemistry and ADMET colleagues, means we can significantly reduce your DMTA cycles and get you from hit to lead in the most cost- and time-effective way.

Cancer cells were seeded either alone or in the presence of fibroblasts. The formed spheroids were then imaged using the IncuCyte imaging platform on days 3 and 6.
Cancer cells were seeded either alone or in the presence of fibroblasts. The formed spheroids were then imaged using the IncuCyte imaging platform on days 3 and 6.
Compounds’ cytotoxic effects were calculated and expressed as EC50: Doxorubicin: A549 - 2D 0.05 μM vs 3D 1.8 μM, BT474 - 2D 0.3 μM vs. 3D n.d. Entinostat: HepG2 - 2D 0.4 μM vs. 3D 0.7 μM, BT474 - 2D 1.0 μM vs. 3D 2.7 μM
Compounds’ cytotoxic effects were calculated and expressed as EC50: Doxorubicin: A549 - 2D 0.05 μM vs 3D 1.8 μM, BT474 - 2D 0.3 μM vs. 3D n.d. Entinostat: HepG2 - 2D 0.4 μM vs. 3D 0.7 μM, BT474 - 2D 1.0 μM vs. 3D 2.7 μM
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