Gene Expression Analysis

Our team of experts specialize in developing, optimizing and validating gene expression readouts across in vitro, in vivo or ex vivo assay systems. Whether you’re investigating mechanism of action (MOA), downstream gene regulation or biomarker pathways, we deliver tailored solutions to advance your research.

DNA/RNA isolation and purification

We employ high-throughput protocols to isolate and purify nucleic acids from diverse cells and tissue. Our methods ensure accurate quality and quantity measurements using fluorescent-based assays and Agilent^® instrumentation setting the foundation for reliable downstream applications.

Quantitative PCR (qPCR)

In drug discovery, understanding the effects of test biologics or small molecules on gene expression can be a key component of your program. Our real-time quantitative PCR (qPCR) service is tailored to meet the unique needs of your project. 

Highly specific and sensitive, qPCR is a versatile high-throughput confirmatory method to validate and quantify gene expression of drug targets, pharmacodynamic (PD) markers, or predictive biomarkers, helping you better understand your compound or target and resolve the molecular mechanism of action.

Our qPCR platform is designed to support drug discovery projects performed with both supplied samples, as well as high quality cellular and animal models, offering a complete in-house service from sample generation through to analysis.

NanoString

NanoString’s nCounter® platform enables highly sensitive and automated analysis of up to 800 RNA or DNA targets simultaneously. By eliminating amplification bias, it bridges the gap between qPCR and Next-Generation Sequencing (NGS). 

We offer off-the-shelf and custom panels for immunology, immuno-oncology and oncology, and neuroscience. This enables determination of signaling pathways and the regulation of downstream gene profiles in challenging sample types such as FFPE, tissue, crude-cell lysates, and biofluids.

In-Situ hybridization (iSH)

Investigate tissue distribution and localization of drug targets or biomarkers with unparalleled specificity and sensitivity. Detect up to 4 RNA targets on the same tissue and combine RNA and protein detection on a single slide for a comprehensive understanding.

In-situ hybridization (iSH) delivers RNA detection and visualization within intact tissue, providing spatial biology context. This enables the investigation of tissue distribution and localisation of drug targets or biomarkers in a highly specific and sensitive manner. We use RNAscope and BaseScope assays on a fully automated Leica BOND autostainer, providing high-quality, high-throughput, and reliable RNA detection.

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